Simoa單分子免疫陣列超靈敏細胞因子
檢測服務
產品介紹：
SimoaTM（Single Molecular Array）又稱作數字式ELISA，是基于Simoa HD-1數字式單分子免疫陣列分析儀，可直接對血清和血漿中蛋白、核酸等生物標志物進行檢測的技術。它的檢測靈敏度比傳統的免疫檢測技術平均高1000倍以上，將蛋白質檢測技術直接帶入單分子、數字化檢測時代，成為fg級超低豐度蛋白質檢測領域的優勢技術。
技術原理：
1、將捕獲抗體偶聯到磁珠上，磁珠與目標抗原結合，目的抗原與檢測抗體結合并帶有梅標記。
2、洗滌磁珠以去除非特異性結合的蛋白，加入酶反應底物。
3、將加入底物的磁珠轉入Simoa光盤上，將熒光信號封閉在小孔內。
應用領域：
Simoa技術可用于腫瘤、神經、感染性疾病、免疫炎癥、miRNA檢測，特別是癌癥的早期檢測、術后的監測和精準用藥，將極大推動精準醫療的發展。運用Simoa可檢測到的低濃度生物標志物進行準確分析，為生命科學研究、體外診斷、伴隨診斷和血液篩查等領域。目前在全球引用該技術發表的文獻有200多篇，包括高分雜志Nature, Nature Biotechnology, JEM, JAMA Neurology等。
參考文獻
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